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Glacial Acetic Acid Molarity: A Guide for Laboratory Use

Glacial acetic acid is a common reagent in organic chemistry and biochemistry. It is a colorless liquid with a pungent vinegar-like odor and a high acidity. It can be used as a solvent, a catalyst, an acetylating agent, and a reactant in various reactions. In this article, we will explain what glacial acetic acid molarity is, how to calculate it, and how to prepare different concentrations of acetic acid from glacial acetic acid.

What is Glacial Acetic Acid Molarity?

Molarity is a unit of concentration that expresses the number of moles of a solute per liter of solution. A mole is a unit of amount that represents 6.022 x 10^23 particles of a substance. Glacial acetic acid is the purest form of acetic acid, with a concentration of about 99.7% by mass and a density of 1.049 g/mL at 25 °C. Therefore, we can say that 1 liter of glacial acetic acid contains 17.416 moles or in other words molarity of glacial acetic acid is equal to 17.416.

How to Calculate Glacial Acetic Acid Molarity?

To calculate the molarity of glacial acetic acid, we need to know its molecular weight and density. The molecular weight of acetic acid is 60.05 g/mol and its density is 1.049 g/mL at 25 °C. Using these values, we can apply the following formula:

Molarity = (mass % x density x 1000) / molecular weight

Plugging in the numbers, we get:

Molarity = (99.7 x 1.049 x 1000) / 60.05

Molarity = 1741.6 / 60.05

Molarity = 29

How to Prepare Different Concentrations of Acetic Acid from Glacial Acetic Acid?

To prepare different concentrations of acetic acid from glacial acetic acid, we need to dilute it with water using a volumetric flask or a graduated cylinder. The amount of glacial acetic acid and water needed can be calculated using the dilution equation:

M1V1 = M2V2

Where M1 and V1 are the molarity and volume of the concentrated solution (glacial acetic acid), and M2 and V2 are the molarity and volume of the diluted solution (acetic acid). For example, if we want to prepare 100 mL of 2 M acetic acid from glacial acetic acid, we can use the following steps:

1) Rearrange the dilution equation to solve for V1:

V1 = (M2V2) / M1

2) Plug in the values:

V1 = (2 x 100) / 17.416

V1 = 11.48 mL

3) Measure 11.48 mL of glacial acetic acid using a pipette or a burette and transfer it to a 100 mL volumetric flask.

4) Add distilled water to the flask until the mark is reached and mix well.

5) Label the flask as 2 M acetic acid and store it in a cool and dark place.

Similarly, we can prepare other concentrations of acetic acid from glacial acetic acid by adjusting the values of M2 and V2 accordingly.

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